Program

 

 

 

 

 

 


 

ApiCOWplexa-APICOMPLEXA IN FARM ANIMALS

4th International meeting - San Lorenzo de El Escorial, Madrid, Spain

 

WEDNESDAY, 11th October 2017-Eurofoum (San Lorenzo de El Escorial, Madrid)

 

14:00

Registration

 

17:00 - 17:15

 

Welcome to Apicowplexa

 

Opening Lecture

Session Chairs: Andrew Hemphill and Alexandre Leitão

 

17:15 - 18:15

Key note (K) 01

 

Apicomplexan parasites: Their relevance for livestock production and veterinary public health. Franz Conraths, Friederich Loeffler Institute, Germany

 

18:15 - 19:15

K 02

 

“Prison Break”. The natural egress of Toxoplasma from infected cells is a tightly programmed event. Dominique Soldati, University of Geneve, Switzerland

 

20:00

 

Welcome reception

 

THURSDAY, 12th October 2017-Eurofoum (San Lorenzo de El Escorial, Madrid)
Systems biology, 8:30 - 10:00

Session Chairs: Dominique Soldati and Jeroen Saeij

Oral communications (0)

8:30 - 8:45

O 01

 

An integrated model of host-parasite interactions in Coccidian parasites, Dong Xia

 

8:45 - 9:00

O 02

 

The three-dimensional ultrastructure of Eimeria tenella sporozoites, Alana Burrell

 

9:00 - 9:15

O 03

 

Transcriptome modulation of bovine trophoblast cells in vitro by Neospora caninum, Pilar Horcajo

 

9:15 - 9:30

O 04

 

Evolution of the micropyle of Eimeria acervulina young oocyst and its assumed role, Jean Michel Reperant

 

9:30 - 9:45

O 05

 

Generation of population genomics data for an intracellular parasite of nucleated mammalian cells, Richard Bishop

 

9:45 - 10:00

O 06

 

Differential gene expression in Neospora caninum, John Ellis

 

10:00 - 10:30

Coffee Break

 

Host-parasite interactions I, 10:30 - 12:30

Session Chairs: Jon Boyle and Fiona Tomley

Invited Speaker

10:30 - 11:15

K 03

 

Toxoplasma proteins that modulate the host cell. Jeroen Saeij, University of California-Davis, United States of America

 

Oral communications  

11:15 - 11:30

O 07

 

A new class of apically confined, transmembrane micronemal proteins play a pivotal role in host cell invasion by Toxoplasma gondii tachyzoites, Furio Spano

 

11:30 - 11:45

O 08

 

Toxoplasma gondii aspartyl protease 3: a microneme and rhoptry protein maturase essential for host plasma membrane lysis during egress and rhoptry discharge during invasion, Sunil Kumar Dogga

 

11:45 - 12:00

O 09

 

miR-126-5p by direct targeting of JNK-interacting protein-2 (JIP-2) plays a key role in Theileria-transformed macrophage virulence, Malak Haidar

 

12:00 - 12:15

O 10

 

In vitro characterization of bovine macrophages infection by Neospora caninum isolates of high and low virulence, Marta García-Sánchez

 

12:15 - 12:30

O 11

 

Virulence and attenuation of Theileria annulata-infected macrophages are stable parasite-associated traits transferable to fresh macrophages of a different MHC type, Gordon Langsley

 

12:30 - 14:00

 

Lunch  

 

Host-parasite interactions II, 14:00 - 15:00

 

Session Chairs: Furio Spano and Julio Benavides

Oral communications

14:00 - 14:15

O 12

 

Cultivation of Hammondia hammondi in vitro reveals new insights into the timing of parasite development that can be exploited for genetic manipulation, Jon Boyle

 

14:15 - 14:30

O 13

 

Experimental Toxoplasma gondii and Eimeria tenella co-infection in chickens, Lysanne Hiob

 

14:30 - 14:45

O 14

 

Neospora caninum infection in a pregnant bovine model at mid gestation: comparison of early infection dynamics between high- (Nc-Spain7) and low- (Nc-Spain1H) virulence isolates, Laura Jiménez-Pelayo

 

14:45 - 15:00

O 15

 

Development of the first in vivo bovine experimental model of chronic besnoitiosis, Carlos Diezma-Díaz

 

Epidemiology and diagnostics I, 15:00 - 16:15

Session Chairs: Luís Gondim and Frank Katzer

Invited Speaker

15:00 - 15:45

K 04

 

Extensive review on potential risk and protective factors for Toxoplasma gondii infection in farm animals: What is remaining after excluding all presumably confounding or effect modifying factors? Gereon Schares, Friederich Loeffler Institute, Germany

 

Oral communications

 

15:45 - 16:00

0 16

 

Analysis of Toxoplasma gondii clonal type-specific antibody reactions in experimentally infected turkeys and chickens by peptide microarray, Pavlo Maksimov

 

16:00 - 16:15

0 17

 

Serotyping of Toxoplasma gondii infections using strain-specific peptides, David Arranz

 

16:15 - 16:45

Coffee Break

 

Epidemiology and diagnostics II, 16:45 - 18:15

 

Session Chairs: Franz Conraths and Michael Reichel

Invited Speaker

16:45 - 17:15

K 05

 

Advances in the diagnosis of bovine besnoitiosis: current options and applications for control. Gema Alvarez-García, University Complutense of Madrid, Spain

 

Oral communications  

17:15 - 17:30

O 18

 

Deciphering ‘cryptic’ Eimeria isolated from the domestic chicken, Fiona Tomley

 

17:30 - 17:45

O 19

 

Multigenome sequence based genotyping of Eimeria species causing coccidiosis in Ontario sheep and goats, Evelin Rejman

 

Invited Speaker  

17:45 - 18:15

K 06 

An overview on Theileriosis: new diagnostic tools and their limits Alessandra Torina, Istituto Zooprofilattico Sperimentale della Sicilia "A.Mirri", Italy

 

18:15 - 19:30

Poster session

 

20:00   

 

Dinner

 

FRIDAY, 13th October 2017-Eurofoum (San Lorenzo de El Escorial, Madrid)

Vaccination and immune responses, 8:30 – 10:00

 

Session Chairs: John Ellis and Ivan Morrison

Invited Speaker

8:30 - 9:15

K 07

 

DC-complexa: species-specific adaptation of dendritic cells to pathogen diversity. Arthur Summerfield, University of Berne, Switzerland

 

Oral communications  

9:15 - 9:30

O 20

 

Analysis of local and peripheral immune response developed in sheep experimentally infected with Toxoplasma gondii at different times of gestation, Julio Benavides

 

9:30 - 9:45

0 21

 

CD103+CD11b- intestinal dendritic cells are critical players for controlling the different steps of C. parvum infection, Fabrice Laurent

 

9:45 - 10:00

O 22

 

Coccidiosis in poultry: development of Eimeria as vaccine vectors to streamline anticoccidial vaccination, Iván Pastor Fernández

 

10:00 - 10:30

Coffee Break

Biosafety and treatment, 10:30 - 12:30

 

Session Chairs: Kayode Ojo and Ronald Kaminsky

Invited Speaker

10:30 - 11:00

K 08

 

Drugs and drug targets in pregnant models for Toxoplasma and Neospora infection. Andrew Hemphill, University of Berne, Switzerland

 

Oral communications  

11:00 - 11:15

O 23

 

Bumped kinase inhibitors and their effects on the host-parasite relationship during experimental N. caninum infection in vitro and in mice, Pablo Winzer

 

11:15 - 11:30

O 24

 

Characterisation of drug-resistance to buparvaquone in Theileria annulata populations in Turkey, Tulin Karagenc

 

11:30 - 11:45

O 25

 

In vitro screening of commercially available anti-coccidials identifies diclazuril and decoquinate as potential therapeutic candidates against Besnoitia besnoiti infection, Alejandro Jiménez-Meléndez

 

11:45 - 12:00

O 26

 

Variations in anticoccidial efficacy on different Eimeria spp. in lambs based on oocyst excretion, Ane Odden

 

Invited Speaker  

12:00 - 12:30

K 09

Advances in bumped-kinase inhibitors for human and animal therapy of cryptosporidiosis. Wes Van Voorish, University of Washington, United States of America

 

 

12:30 - 14:00

Lunch 

 

Food and waterborne zoonosis, 14:00 – 15:45

 

Session Chairs: Gereon Schares and Lee Innes

Invited Speaker

14:00 - 14:45

K 10

 

Food and Waterborne Protozoa: a veterinary and public health perspective, Frank Katzer, Moredun Research Institute, United Kingdom

 

Oral communications  

14:45 - 15:00

O 27

 

The impact of vegetation on the environmental transfer of Cryptosporidium oocysts, Claire E. Paton

 

15:00 - 15:15

O 28

 

In vitro host cell viability as putative correlate of virulence of Cryptosporidium parvum field isolates in Eastern Germany, Ivette Holzhausen

 

15:15 - 15:30

O 29

 

Impact of confinement housing on study end-points in the calf model of cryptosporidiosis, Jennifer Zambriski

 

15:30 - 15:45

O 30

 

Host-pathogen interactions in neonatal calves naturally and experimentally infected with Cryptosporidium parvum, Sarah Thomson

 

15:45 - 16:15

Coffee Break

 

3 minutes oral poster presentations (P), 16:15 - 17:15

Session Chairs: Alexandre Leitão and Tulin Karagenęž”

P 1. Comparative study between pregnant mouse interference test and zebrafish embryo acute toxicity test: a possible replacement in anti-parasitic chemotherapy trials?, Nicoleta Anghel

P 2. Investigating the virulence of Toxoplasma gondii isolates from Brazil and Saint Kitts in mice, Lauren E. Black

P 3. Retrospective molecular diagnosis of Neospora caninum in bovine aborted fetus in Uruguay: Preliminary results, Briano Carolina

P 4. Isolation of a Neospora caninum goat strain from Southern Minas Gerais, Brazil, Rafael C. Costa

P 5. A component of the core kinase module of MEN and Hippo pathways, Mob1 is a critical factor in Toxoplasma gondii replication, Inés Delgado

P 6. Cytokine response to Cystoisospora suis infections in immune competent pigs, Barbara Freudenschuss

P 7. Transcriptional analysis of bovine monocyte-derived macrophages infected with high and low virulent isolates of Neospora caninum, Marta García-Sánchez

P 8. Phylogenetic diversity of Eimeria spp. in different genotypes of house mice (Mus musculus) from the European Hybrid Zone using a multiple marker approach, Víctor Hugo Jarquín-Díaz.

P 9. Host-parasite interactions of N. caninum isolates of different virulence in bovine fetal and maternal placental cell lines, Laura Jiménez-Pelayo

P 10. HSP81.2 from Arabidopsis thaliana enhances the immune response against NcSAG1 from Neospora caninum protein and partially protects mice from congenital neosporosis, Sofia A. Bengoa-Luoni

P 11. Effects of Eimeria tenella infection on chicken caecal microbiome diversity, exploring variation associated with severity of pathology, Sarah Macdonald

P 12. Dose-titration of virulent Neospora caninum isolate Nc-Spain7 in pregnant sheep at 90 days of gestation, Roberto Sánchez

P 13. A reduction in weight gain in beef calves with clinical cryptosporidiosis, Hannah Jade Shaw

 

Public engagement and knowledge exchange, 17:15 - until finishing the discussion

How to raise awareness for ruminant apicomplexan (open round table)

Chairman: Ronald Kaminsky, Consulting for Parasitology and Drug Discovery

Participants: Fiona Tomley, RVC, Wes VanVoorhis, U-Washington, Peter Opdam, MSD and Darrell Klug, Senior Consultant, Brakke Consulting, Inc.

 

20:00

Conference dinner (by registration)

 

Poster session

 

P 14. Microgametes – just motile nuclei or pivotal stages of the life cycle of Cystoisospora suis?, Anja Joachim

P 15. Demonstration of the presence of T. gondii in bio pigs intended for human consumption in Belgium, Ignacio Gisbert-Algaba

P 16. Analysis of allelic diversity of two immunodominant antigen genes of T. annulata and T. lestoquardi in Oman, Salama Al-Hamidhi

P 17. Genetics differentiation of T. lestoquradi in Africa and Asia, Hamza Babiker

P 18. Role of wildlife in the transmission of Cryptosporidium parvum to humans and livestock, Ross Bacchetti

P 19. Anti-cancer drugs affecting apicomplexan parasites: characterization of novel ruthenium-based compounds and their effects on Toxoplasma gondii, Vreni Balmer

P 20. Semi-high throughput screening of the Pathogen Box for inhibitors with dual efficacy against Giardia lamblia and Cryptosporidium parvum, Lynn Barrett

P 21. Evaluating the impact of pregnancy-associated immunomodulation on specific immune mechanisms against Neospora caninum infection in mice, Afonso P. Basto

P 22. Can multi-copy genes be an alternative for the diagnosis of tbds in cattle? Huseyin Bilgin

P 23. Differential modulation of the Golgi and endosomal system in host cells infected with Besnoitia besnoiti, Rita Cardoso

P 24. A new indirect ELISA for the detection of Besnoitia besnoiti antibodies in individual and bulk milk samples, Loic Comtet

P 25. The IDScreen® Besnoitia indirect 2.0 serum ELISA perfectly correlates with confirmatory techniques, Loic Comtet

P 26. Experimental infection with Besnoitia besnoiti tachyzoites in calves and young bulls, Carlos Diezma-Díaz

P 27. A new ELISA test to diagnose Besnoitia spp. infection in bovids and wild ruminants with improved specificity avoiding the use of a confirmatory test, Gema Álvarez-García

P 28. Development and characterization of monoclonal antibodies against Besnoitia besnoiti tachyzoites, Gema Álvarez-García

P 29. Serological and molecular epidemiology of Toxoplasma gondii infection in intensive pig farms in Northern Italy, Alessia Libera Gazzonis

P 30. Sarcocystis neurona and Neospora caninum in Brazilian opossums (Didelphis spp.): Molecular investigation and in vitro isolation of Sarcocystis spp., Luís Gondim

P 31. Development of an alternative assay to study the infectivity of T. gondii, Tina Goroll

P 32. Integrative transcriptome and proteome analyses define marked differences between N. caninum isolates throughout the tachyzoite lytic cycle, Javier Regidor-Cerrillo

P 33. Toxoplasma gondii infections in stranded marine mammals in France and Romania, Radu Blaga

P 34. Immune response induced by the Mic1-3 Knockout Toxoplasma gondii vaccine strain in the parasite definitive feline host, Radu Blaga

P 35. Genus-specific antibodies for the diagnosis of Neospora caninum and Toxoplasma gondii using Immunohistochemistry in abortion cases of ruminants, A.I. Macrae

P 36. Characterization of Theileria equi antigen infecting Donkeys in Egypt, Olfat A. Mahdy

P 37. Coccidian in Oryctolagus cunniculus from Tenerife, Canary Islands, Spain, Natalia Martín Carrillo

P 38. Carotenoid-enriched corn protects poultry against coccidiosis, Beatriz Serrano-Pérez

P 39. Toxoplasma inhibitor of STAT1 transcription effector protein IST and its relevance in virulence for closely related Neospora caninum and Hammondia hammondi, Philipp Olias

P 40. Anticoccidial effect of naringenin and grape fruit peel extract in growing lambs naturally-infected with Eimeria spp, María Cristina Guerrero Molina

P 41. Microsatellite analysis reveals high diversity among geographically close isolates of Cystoisospora suis, Baerbel Ruttkowski

P 42. Chicken line-dependent mortality after experimental infection with three type IIxIII recombinant Toxoplasma gondii clones, Gereon Schares

P 43. Immune -endocrine patterns in dairy cattle experimentally infected with N. caninum in the second trimester of gestation, Beatriz Serrano

P 44. Development of a recombinant protein based indirect ELISA for the detection of serum antibodies against Cystoisospora suis in swine, Aruna Shrestha

P 45. Deciphering the host cues responsive circadian transcriptome of apicomplexan parasite Plasmodium chabaudi, Amit Kumar Subudhi

P 46. Analysis of Babesia bigemina Ap+C7:C67ical Membrane Antigen-1 immunogenicity and its characterization in Apicomplexa, Alessandra Torina

P 47. Distribution of cattle babesiosis in Palermo province (Sicily), Alessandra Torina

P 48. Serological study of Toxoplasma gondii and Neospora caninum in a wildlife conservation area in southern Portugal, Alexandre Leitão

P 49. Evaluation of Eimeria sp. infection and predictors of oocysts excretion in newly introduced beef cattle in northern Italy, MT Manfredi

P 50. Mutual influences of the apicomplexan parasites Toxoplasma gondii and Eimeria tenella in poultry macrophages, Runhui Zhang

P 51. Efficacy of VFO-IS-01, a live attenuated immunostimulant against Salmonella enteritidis, Eimeria acervulina and Influenza H7N1 infections in chicks, Mehdi Ellouze

P 52. Effects of challenge dose and inoculation route of virulent Neospora caninum Nc-Spain7 isolate in pregnant cattle at mid gestation, Patricia Vazquez

P 53. Significant reduction of Neospora caninum vertical transmission and postnatal mortality by a toll-like-receptor 2-targeting vaccine formulation in the pregnant mouse model of neosporosis, Adriana Aguado

P 54. Evidence that transfer of Theileria annulata parasites from infected to uninfected leukocytes occurs by spontaneous cell fusion, Ivan Morrison

P 55. Placental lesions associated with abortions and stillbirths in goats naturally infected with Neospora caninum from Southern Minas Gerais, Brazil, Rafael C. Costa

P 56. Toxoplasma gondii tubulin-binding cofactor B a polarity factor required for host cell invasion and replication, Inés Delgado

P 57. Pharmacokinetics, safety and efficacy of Bumped Kinase Inhibitor (BKI) 1553 in a pregnant sheep model of neosporosis, Roberto Sánchez

P 58. Cryptosporidium infections among animals and humans in Greece, Smaragda Sotirak

P 59. Cryptosporidium parvum increases intestinal permeability through interaction with epithelial cells and IL-1? and TNF? released by inflammatory monocytes, Fabrice Laurent

P 60. Investigation of tick-borne disease in Indian bovines: breed resistance and transmission blocking as strategies for improved productivity, Stephen  Larcombe

P 61. The relationship between presence of antibodies and direct detection of Toxoplasma gondii in slaughtered cattle from four European countries, M. Opsteegh

   

                       

 

  apicowplexa2017@gmail.com